Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07
نویسندگان
چکیده
منابع مشابه
Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07
BACKGROUND The rising concerns about the scarcity of fossil fuels, the emission of green house gasses and air pollution by incomplete combustion of fossil fuel have also resulted in an increasing focus on the use of cellulases to perform enzymatic hydrolysis of the lignocellulosic materials for the generation of bioethanol. The aim of this study was to isolate a potential thermo-solvent toleran...
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A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on S...
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A total of five bacterial strains were isolated from soil samples of Selakui area of Dehradun, India, and were subjected to screening for their carboxy methyl cellulose (CMC) hydrolysis activity. Results revealed that, MJSH 1205 have maximum exocellulase activity and after fermentation at temperature 27°c, pH 7 and 1.5 % CMC concentration and 0.5% of urea as nitrogen source, bacterial strain MJ...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2015
ISSN: 1472-6750
DOI: 10.1186/s12896-015-0129-9